Modulation of cutaneous wound healing by ozone: differences between
young and aged mice.
Department of Internal Medicine, School of
Medicine, UC Davis, CA, USA.
Cutaneous tissues are frequently exposed to
prooxidative environments, including UV radiation and air pollutants.
Among the latter, ozone (O(3)) is of particular concern because of its
high and dominating presence in photochemical smog. It is well known
that O(3) depletes small molecular weight antioxidants, oxidizes
proteins, induces lipid peroxidation and activates cellular responses in
various tissues. Using an in vivo model (SKH-1 hairless mice), the
interaction between O(3) exposure (0.5ppmx6h/day) and age was examined
in relation to cutaneous wound healing. Compared to younger (8 weeks)
mice, older (18 months) mice exposed to O(3) (day 0 to day 9 after
wounding) exhibited delayed wound closure, increased lipid peroxidation
(measured as 4-HNE protein adducts) and protein oxidation (measured as
carbonyls concentration) and decreased levels of P-IkappaBalpha and
TGFbeta protein. These findings support the hypothesis that oxidant
pollutant exposure and age interact so as to disrupt normal wound
PMID: 16129572 [PubMed - indexed for MEDLINE]