Aflatoxicosis in turkey poults is prevented by treatment of
naturally contaminated corn with ozone generated by electrolysis.
Department of Veterinary Anatomy and Public Health,
Faculty of Toxicology, College of Veterinary Medicine, Texas A&M
University, College Station 77843-4458, USA.
Previous studies have demonstrated that a novel source
of ozone gas (O3) maybe used to chemically degrade numerous mycotoxins,
including aflatoxin (AF) B1. Subsequent in vitro analyses demonstrated
detoxification of AFB1, suggesting a potential method of remediate
AF-contaminated grain. The objective of this study was to evaluate the
capability of electrochemically produced ozone to degrade AFB1 in
naturally contaminated whole kernel corn and confirm detoxification in
turkey poults. Corn was procured from the southern coastal areas of
Texas and HPLC revealed 1,220 +/- 73.3 ppb AFB1. Control and
contaminated corn were treated for 92 h with O3 at 200 mg/min in 30 kg
batches; greater than 95% reduction of AFB1 in contaminated corn was
achieved. One-day-old female turkey poults were fed 1) control corn, 2)
control corn + O3, 3) AFB1 corn, or 4) AFB1 corn + O3 mixed in rations
(46% by wt.) and consumed ad libitum for 3 wk. When compared with
controls, turkeys fed AFB1 corn had reduced body weight gain and
relative liver weight, whereas turkeys fed control corn + O3 or AFB1
corn + O3 did not differ from controls. Furthermore, alterations in the
majority of relative organ weight, liver discoloration, serum enzyme
activity, hematological parameters, and blood chemistry caused by AFB1
were eliminated (no difference from controls) by treatment with O3.
These data demonstrate that treatment of contaminated corn with
electrochemically produced O3 provided protection against AFB1 in young
turkey poults. It is important to note that treatment of control corn
with O3 did not alter the performance of the turkey poults.
PMID: 9706072 [PubMed - indexed for MEDLINE]