Genotoxic potential of by-products in drinking water in relation to
water disinfection: survey of pre-ozonated and post-chlorinated drinking
water by Ames-test.
Department of Public Health, Semmelweis University,
P.O. Box 370, Budapest H-1445, Hungary.
Mutagenic potential of drinking water samples derived
from ranneywells was studied. 100-100 l of untreated (rough) and
ozone-treated as well as chlorinated-disinfected water were dropped on
and adsorbed by macroreticular resin columns (Serdolit PAD-III and
Amberlite XAD-2). The adsorbed material was desorbed by methanol and
dichloromethane. After elimination of the solvents by vacuum
distillation the adsorbed material was dissolved in dimethylsulfoxide.
The mutagenic activity was tested in the Ames-Salmonella/rat liver
microsome system. The tester strains were TA-98 and TA-100. The material
adsorbed to Serdolit PAD-III from rough and also disinfected water did
not induce mutagenicity in case of the TA-98 tester strain, irrespective
of activation by liver microsomes. However, the material adsorbed to
Amberlite XAD-2 exerted mutagenic effect on the TA-98 tester strain,
with and without liver microsome activation, both in case of rough and
disinfected water. The TA-100 tester strain showed mutation after, but
not without activation, when treated with the material adsorbed by
either Serdolit PAD-III or Amberlite XAD-2, in case of rough water.
Material derived from disinfected water and adsorbed to Serdolit
PAD-III, caused mutation of the TA tester strain also only after
activation. The material derived from disinfected water and adsorbed to
Amberlite XAD-2 proved to be mutagenic to the TA-100 tester strain both
without and after activation. Mutagenic activity was exerted by the
amount of concentrates derived from 0.28 to 0.83 l of rough and
0.83-2.5l of disinfected water. The mutagenic activity of drinking water
raises the possibility of carcinogenic effect, too. Search for
alternative methods of water disinfection may be recommended.
PMID: 16364533 [PubMed - indexed for MEDLINE]